Peripheral mechanisms of nociception in the musculoskeletal system, Part II (rev230616FH)
The aim is to determine pathogenic mechanisms of musculoskeletal pain caused by low levels of mechanical exposures. In spite of many years of research the causal link between work and musculoskeletal pain disorders remains elusive. Only for some acute overuse syndromes and high-level static loads are the pathogenesis largely known. Knowledge of causation is essential for prevention and interventions at the workplace. The methods developed in this project will be employed in studies of effects of work-related mechanical exposures.
This experiment is designet to develop and optimize a bioassay of the physiological and functional significance of mediating and modifying factors of nociceptor activation during Low Intensity Working Activities (LIWA) and pain using primary cultures of dissociated sensory Dorsal Root Ganglion (DRG) neurons from 140 adult rats. The animals will be healthy and unaffected until sacrifice under deep gas anesthesia. These cell experiments reduce the necessity of in vivo experiments and the number of animals, because many conditions can be tested in cells from one animal.
This experiment is designet to develop and optimize a bioassay of the physiological and functional significance of mediating and modifying factors of nociceptor activation during Low Intensity Working Activities (LIWA) and pain using primary cultures of dissociated sensory Dorsal Root Ganglion (DRG) neurons from 140 adult rats. The animals will be healthy and unaffected until sacrifice under deep gas anesthesia. These cell experiments reduce the necessity of in vivo experiments and the number of animals, because many conditions can be tested in cells from one animal.