Effects of salmon ploidy on susceptibility to viral diseases
Begrunnelse for etterevalueringen
The Norwegian Food Safety Authority must retrospectively assess all severe experiments.
Etterevaluering
Pilot PRV1 infection trial in diploid, triploid, and mosaic post-smolt salmon (produced with 0, 9500 psi, or 6500 psi pressure treatment, respectively) showed interesting results – with some surprising trend in viral load level and growth performance that can be correlated to ploidy level. However, statistical significance was only observed in some treatment groups due to use of less experimental fish. It should also be noted that random mortality was observed during transport and acclimatization (prior to start of PRV1 challenge), which resulted to lower number of experimental fish as initially planned. Thus, further study is necessary to confirm any of our observed trends.
Smaller number of experimental fish was used because of the limited availability of experimental fish with different ploidy status at the time of the scheduled PRV1 challenge (Mock infection: 40 fish total; PRV1 infection: 42 fish total). No increase in severity was observed during the actual challenge regardless of ploidy status of experimental fish infected with PRV1.
Ethics and rules in the handling and use of experimental fish were strictly followed in this study. No additional refinement of existing protocols is necessary.
Smaller number of experimental fish was used because of the limited availability of experimental fish with different ploidy status at the time of the scheduled PRV1 challenge (Mock infection: 40 fish total; PRV1 infection: 42 fish total). No increase in severity was observed during the actual challenge regardless of ploidy status of experimental fish infected with PRV1.
Ethics and rules in the handling and use of experimental fish were strictly followed in this study. No additional refinement of existing protocols is necessary.