Evaluation of novel 18F-FAPI tracer for PET imaging of cancer associated fibroblasts
1 Purpose
The purpose of the experiment is to evaluate a novel 18F-labelled PET radioligand (18F-FAPI) for non-invasive imaging of FAP expression in cancer models. specifically targeting tumor microenvironment.
2 Distress
Animals will be inoculated with a human cancer cell lines subcutaneously, subjected to a single longitudinal PET/CT scan and sacrificed thereafter. Overall, the severity grade is accessed to be moderate, in line with "Forskrift for forsøk med dyr".
3 Expected benefit
Currently, 18F-FDG is a gold standard tracer used for cancer detection. Uptake of the 18F-FDG is based on increased metabolic rate of the cancer cells, so called Warburg effect. However, many cancers do not possess those properties, appearing as negative on PET scans. This leads to falsely identification of such patients as tumor free. The proposed tracer will add a new tool for cancer detection into the clinical practice that will help physicians to identify previously negative tumor lesions.
4 Number of animals, and what kind
We shall use 30 male BALB/c nude mice. 15 mice will be used for xenografting with the use of A549-FAP cells lines and 15 mice will be use for xenografting with HT-1080-FAP cells
5 How to adhere to 3R
Multiple in vitro studies will be conducted to prove the efficacy, characterization and specificity in all
2 cell lines before the conduction of the in vivo experiments. We have already utilized all 2 models in vivo, and have good knowledge of this models about group size, timing of treatment etc.. The technicians conducting the study have long experience with similar studies using well-refined techniques.
We would like to see gender difference in terms of development of xenograft and 18F-FAPI tracer expression . Therefore we want to use both Male and Female.
The purpose of the experiment is to evaluate a novel 18F-labelled PET radioligand (18F-FAPI) for non-invasive imaging of FAP expression in cancer models. specifically targeting tumor microenvironment.
2 Distress
Animals will be inoculated with a human cancer cell lines subcutaneously, subjected to a single longitudinal PET/CT scan and sacrificed thereafter. Overall, the severity grade is accessed to be moderate, in line with "Forskrift for forsøk med dyr".
3 Expected benefit
Currently, 18F-FDG is a gold standard tracer used for cancer detection. Uptake of the 18F-FDG is based on increased metabolic rate of the cancer cells, so called Warburg effect. However, many cancers do not possess those properties, appearing as negative on PET scans. This leads to falsely identification of such patients as tumor free. The proposed tracer will add a new tool for cancer detection into the clinical practice that will help physicians to identify previously negative tumor lesions.
4 Number of animals, and what kind
We shall use 30 male BALB/c nude mice. 15 mice will be used for xenografting with the use of A549-FAP cells lines and 15 mice will be use for xenografting with HT-1080-FAP cells
5 How to adhere to 3R
Multiple in vitro studies will be conducted to prove the efficacy, characterization and specificity in all
2 cell lines before the conduction of the in vivo experiments. We have already utilized all 2 models in vivo, and have good knowledge of this models about group size, timing of treatment etc.. The technicians conducting the study have long experience with similar studies using well-refined techniques.
We would like to see gender difference in terms of development of xenograft and 18F-FAPI tracer expression . Therefore we want to use both Male and Female.