Test the efficacy of compounds v,w and x against pathogen L in Atlantic salmon (copy)
1 Formål
The objective of the trials included under this application is to document the efficacy and pharmacokinetics of selected therapeutic compounds against pathogen L in Atlantic salmon.
2 Skadevirkninger
The experiment will be classified as mildly stressful overall as the study involves infestation with a restricted number of pathogen L over a maximum of 4 weeks which can be considered moderately stressful (mild stress over a long period as per forsøksdyrforskriften Vedlegg B Del 1). It is expected that only a small group of fish (control) will be moderately stressed with pathogen L as other groups will be treated approximately 3 weeks in to the trial. Additionally, the compounds tested here and their family chemicals have already been tested in salmon through bath and feed and did not elicit any safety concerns. This means that the experiment should be classified as moderately stressful.
3 Forventet nytteverdi
Pathogen L is a persistent threat to the salmon farming sector and results in large scale economic losses. Additionally, animal welfare is also compromised with high load of pathogen L. It is expected that, if found promising in this study, these test compounds could be further developed as potential therapeutic agents against pathogen L.
4 Antall dyr og art
320 Atlantic salmon
5 Hvordan etterleve 3R
ERSTATNING: An effect study on live fish needs to be done because the pathogen L can behave or respond differently when infecting fish as opposed to an invitro experiment where they are deprived from their natural host and source of feed/ nutrition.
REDUKSJON: Total fish numbers are calculated based on approximate fish numbers of about 10 fish required per sampling. The objective of the experiment is to identify near to 100% efficacy dose and therefore a sample size based on statistical method has not been used. The fish numbers are considered enough based on previous experiments with same compounds.
FORBEDRING: Fish used in the study will be vaccinated against relevant opportunistic infections that arise primarily due to handling. Additionally, fish will be euthanized by anesthetic overdose for sampling . Fish will be handled gently according to internal SOPs. Mortality is not an outcome of the study and any adverse effect of medication is not expected as much lower doses (than used previously) are used and therefore it is assumed that only general humane endpoints associated with fish husbandry will need to be implemented on the rare occasions of for example fish that does not grow well (become extremely stunted) or begin to develop sores in which case it will be removed and euthanized as early as possible.
The objective of the trials included under this application is to document the efficacy and pharmacokinetics of selected therapeutic compounds against pathogen L in Atlantic salmon.
2 Skadevirkninger
The experiment will be classified as mildly stressful overall as the study involves infestation with a restricted number of pathogen L over a maximum of 4 weeks which can be considered moderately stressful (mild stress over a long period as per forsøksdyrforskriften Vedlegg B Del 1). It is expected that only a small group of fish (control) will be moderately stressed with pathogen L as other groups will be treated approximately 3 weeks in to the trial. Additionally, the compounds tested here and their family chemicals have already been tested in salmon through bath and feed and did not elicit any safety concerns. This means that the experiment should be classified as moderately stressful.
3 Forventet nytteverdi
Pathogen L is a persistent threat to the salmon farming sector and results in large scale economic losses. Additionally, animal welfare is also compromised with high load of pathogen L. It is expected that, if found promising in this study, these test compounds could be further developed as potential therapeutic agents against pathogen L.
4 Antall dyr og art
320 Atlantic salmon
5 Hvordan etterleve 3R
ERSTATNING: An effect study on live fish needs to be done because the pathogen L can behave or respond differently when infecting fish as opposed to an invitro experiment where they are deprived from their natural host and source of feed/ nutrition.
REDUKSJON: Total fish numbers are calculated based on approximate fish numbers of about 10 fish required per sampling. The objective of the experiment is to identify near to 100% efficacy dose and therefore a sample size based on statistical method has not been used. The fish numbers are considered enough based on previous experiments with same compounds.
FORBEDRING: Fish used in the study will be vaccinated against relevant opportunistic infections that arise primarily due to handling. Additionally, fish will be euthanized by anesthetic overdose for sampling . Fish will be handled gently according to internal SOPs. Mortality is not an outcome of the study and any adverse effect of medication is not expected as much lower doses (than used previously) are used and therefore it is assumed that only general humane endpoints associated with fish husbandry will need to be implemented on the rare occasions of for example fish that does not grow well (become extremely stunted) or begin to develop sores in which case it will be removed and euthanized as early as possible.