Marine oils, their biodistribution and effect on health
1 Formål
The aim of this study is to verify increased uptake of marine, essential, oils to the brain. We will do this by performing a biodistribution study in rats, where they will be fed the oil for 5 weeks. During the study we will monitor the amount of oil in blood and after the study is finished the amount of oil will be quantified in several organs, including the brain. Additionally, we will quantify inflammatory markers and the expression of specific neuronal proteins important for the regeneration of brain function.
2 Skadevirkninger
The animals will be fed, and blood samples will be collected. These are quick procedures, and we do not expect them to cause significant pain to the animals (lett belastende), but if necessary, they can be performed under gas anaesthesia. In the time between interventions the animals will be kept in their usual surroundings together with the other rats in the cage, with controlled environment and enrichment which as standard at the department of Comparative medicine; with "strø", nesting material and hiding place, as well as free access to food and water. The animals will be monitored regularly to look for signs of distress or pain. In case the animals are anesthetized for blood sampling, they will be kept on a heated surface to maintain stable body temperature, and respiration will be monitored and the eyes will be kept moist.
3 Forventet nytteverdi
This oil is expected to become a nutraceutical product within a short time frame. If this study and future studies planned are positive, it could reduce the use of anti-inflammatory substances related to cardiovascular disease, reduce the cognitive decline of elderly and speed up recovery from traumatic brain damage.
4 Antall dyr og art
In the study 60 male rats are included.
5 Hvordan etterleve 3R
We always strive to include the 3R's in all our work. In this case we are performing clinical studies in parallel that will supply the information from this study. Also, we will initially include 2/3 of the animals in the study, and if statistical power is achieved, we will not proceed with the remaining 1/3. The study is not very demanding on the animals, they are fed safe products with no anticipated negative effects. Only the most promising oils will be used, based on previous experiments and knowledge. There are no other good alternatives for studying biodistribution in vivo.
The aim of this study is to verify increased uptake of marine, essential, oils to the brain. We will do this by performing a biodistribution study in rats, where they will be fed the oil for 5 weeks. During the study we will monitor the amount of oil in blood and after the study is finished the amount of oil will be quantified in several organs, including the brain. Additionally, we will quantify inflammatory markers and the expression of specific neuronal proteins important for the regeneration of brain function.
2 Skadevirkninger
The animals will be fed, and blood samples will be collected. These are quick procedures, and we do not expect them to cause significant pain to the animals (lett belastende), but if necessary, they can be performed under gas anaesthesia. In the time between interventions the animals will be kept in their usual surroundings together with the other rats in the cage, with controlled environment and enrichment which as standard at the department of Comparative medicine; with "strø", nesting material and hiding place, as well as free access to food and water. The animals will be monitored regularly to look for signs of distress or pain. In case the animals are anesthetized for blood sampling, they will be kept on a heated surface to maintain stable body temperature, and respiration will be monitored and the eyes will be kept moist.
3 Forventet nytteverdi
This oil is expected to become a nutraceutical product within a short time frame. If this study and future studies planned are positive, it could reduce the use of anti-inflammatory substances related to cardiovascular disease, reduce the cognitive decline of elderly and speed up recovery from traumatic brain damage.
4 Antall dyr og art
In the study 60 male rats are included.
5 Hvordan etterleve 3R
We always strive to include the 3R's in all our work. In this case we are performing clinical studies in parallel that will supply the information from this study. Also, we will initially include 2/3 of the animals in the study, and if statistical power is achieved, we will not proceed with the remaining 1/3. The study is not very demanding on the animals, they are fed safe products with no anticipated negative effects. Only the most promising oils will be used, based on previous experiments and knowledge. There are no other good alternatives for studying biodistribution in vivo.