Prevention of obesity-related insulin resistance in mice by anti-obesity treatments
Main goal: to prevent insulin resistance in obese mice by interventions preventing adipose tissue expansion. Obesity is induced by feeding normal mice a high-fat diet (HFD) over a 12 wk period, followed by an 8 wk feeding period during which time the animals will be treated with Calanus oil. Mice fed normal chow is used as lean controls. One week prior to the end of the feeding period, we will determine insulin- and glucose tolerance. Furthermore, blood and tissue samples are taken for analysis of metabolic parameters and gene expression.
Expected effects on the animals: Previous experience has demonstrated less fat deposition, as well as in high fat-fed mice following dietary supplementation of the diet with Calanus oil.
Expected scientific and or social utility value: Obesity is associated with insulin resistance and
cardiovascular disease. Hence, interventions which can prevent the development obesity will be important to combat these conditions.
Number of animals: 48
The feeding/treatment schedule follows a strict plan, which allows maximum utilization of the animals.The mice will be observed closely, and in case of any suffering, distress and lasting harm, we will stop the treatment and not include new animals in these groups.
Expected effects on the animals: Previous experience has demonstrated less fat deposition, as well as in high fat-fed mice following dietary supplementation of the diet with Calanus oil.
Expected scientific and or social utility value: Obesity is associated with insulin resistance and
cardiovascular disease. Hence, interventions which can prevent the development obesity will be important to combat these conditions.
Number of animals: 48
The feeding/treatment schedule follows a strict plan, which allows maximum utilization of the animals.The mice will be observed closely, and in case of any suffering, distress and lasting harm, we will stop the treatment and not include new animals in these groups.