Protection against Edwardsiella and Aeromonas in zebrafish

Aeromonas hydrophila and Edwardsiella sp. are a Gram-negative bacteria that causes hemorrhagic septicemias and mortality in different fish species, including different cichlids and at different growth stages. Vaccination has proved to be an effective disease preventive strategy with ability to reduce disease outbreaks and although it has been shown that injectable vaccines can elicit immune responses that raise protective immunity in target and model species, there is still need for improvement of vaccines particularly the development of new formulations with improved (lessen) side effect profiles. In this study we will explore the use of various nanoparticle formulations for delivery of inactivated vaccines (for injection) in a model system, adult zebrafish, and at the same time study antigen distribution post immunisation.