The effect of copolymer scaffolds functionalized with nano-bioactive molecules on regenerating of critical-sized calvarial bone defect in osteoporotic rats
The research goal of this project is to develop state-of-the-art biomaterials for bone transplantation that will stimulate rapid bone regeneration. There is a clinical need for development and optimizing synthetic scaffolds to repair large bone defects as well as improve bone healing especially in compromised patient with osteoporosis. The novel approach uses plant-derived polysaccharides, mainly Rhamnogalacturonan-I pectins (RG-I), which provide the possibility to both enhance osteogenesis and limit inflammation at the surgical, graft site. RG-I provide considerable flexibility in the delivery format being used as nano-coatings. The aim is to test the effect of 3D copolymer scaffolds with or without nano-bioactive molecules derived from plants on bone regeneration.
Rat calvarial bone defects will be used in Wistar rats after the induction of osteoporosis. The autogenous bone graft will be used as a “gold standard” to reappear bone defects.
In vitro experiments have been extensively studied with regard to this molecule alone and in addition to being incorporated in scaffolds. The need to evaluate its efficacy in regenerating bone in a immunocompromised or inflammatory environment is essential for clinical applications. This can only be done in vivo in an actual bone defect. We will use 2 scaffolds in the same animal in an attempt to reduce the number of animals used
A series of in vitro and in vivo studies have been carried out in our group regarding bone tissue engineering. The methods have been tested and the bone defect model has been well-performed in our previous studies, e.g. (4903, 6866).
Rat calvarial bone defects will be used in Wistar rats after the induction of osteoporosis. The autogenous bone graft will be used as a “gold standard” to reappear bone defects.
In vitro experiments have been extensively studied with regard to this molecule alone and in addition to being incorporated in scaffolds. The need to evaluate its efficacy in regenerating bone in a immunocompromised or inflammatory environment is essential for clinical applications. This can only be done in vivo in an actual bone defect. We will use 2 scaffolds in the same animal in an attempt to reduce the number of animals used
A series of in vitro and in vivo studies have been carried out in our group regarding bone tissue engineering. The methods have been tested and the bone defect model has been well-performed in our previous studies, e.g. (4903, 6866).