NutriDrug Biodistribution
The purpose of the experiments is to evaluate if a drugs and/or nutrients can be formulated in a fish-oil-containing drink to increase uptake in the intestine and also lead to altered and improved biodistribution. 3-4 different drugs or nutrients will be mixed into the patented formulation and fed to mice using oral gavage. After 15, 30, 60, 120 ,240, 360 minutes and 12 and 24 hours, animals will be euthanized and organs and feces will be collected for quantification of the various substances through mass spec imaging.
The goal of the project is to develop a formulation that can contain various types of drugs such as antibiotics, cytostatics and pain killers and can be given to patients in stead of pills or IV injections. It has previously been shown that the taste of fish oil is very well hidden in the formulation.
The study will contain 3(4) groups, with 3 different treatments and one animal being used untreated.
Groups:
1: Control with all substances, but without the final mixing step that forms the stable formulation.
2: Control with the drugs/Pharmaceuticals only.
3: All substances in the complete formulation.
4: No treatment (1 animal).
This is a pilot experiment to look for early indications of improved uptake and altered biodistribution and we plan to use only one animal/timepoint/group. (8+8+8+1 =25 animals) However, in order to open for repetition in case of unclear results, we apply for another 25 animals to repeat experiments if necessary. Hence a total of 50 animals, female balb/c mice is applied for.
The setup of the experiment takes account of the Three R's, especially by starting with only one animal in each group and only repeat datapoints that are either out of pattern or uncertain. Replacement is not feasible because we aim to study biodistriubtion and uptake in the intestine.
The goal of the project is to develop a formulation that can contain various types of drugs such as antibiotics, cytostatics and pain killers and can be given to patients in stead of pills or IV injections. It has previously been shown that the taste of fish oil is very well hidden in the formulation.
The study will contain 3(4) groups, with 3 different treatments and one animal being used untreated.
Groups:
1: Control with all substances, but without the final mixing step that forms the stable formulation.
2: Control with the drugs/Pharmaceuticals only.
3: All substances in the complete formulation.
4: No treatment (1 animal).
This is a pilot experiment to look for early indications of improved uptake and altered biodistribution and we plan to use only one animal/timepoint/group. (8+8+8+1 =25 animals) However, in order to open for repetition in case of unclear results, we apply for another 25 animals to repeat experiments if necessary. Hence a total of 50 animals, female balb/c mice is applied for.
The setup of the experiment takes account of the Three R's, especially by starting with only one animal in each group and only repeat datapoints that are either out of pattern or uncertain. Replacement is not feasible because we aim to study biodistriubtion and uptake in the intestine.