Characterization of Alkbh1-knockout mice
Purpose of this study is to elucidate the physiological function of Alkbh1 in vivo. Therefore we will use a knockout mouse (KO) model in which the Alkbh1 gene is disrupted by insertion of a premature stop codon in exon 7 using the Crisper/Cas method.
Various organs from adult mice of different ages will be taken out for tissue sections and biochemical analysis.
We will establish MEF cells to complement our studies and reduce the number of mice needed.
We have planned our studies based on our previous experience to use the least amount of mice possible. Based on our calculations we will use a maximum of 556 mice in our studies.
The mice will not be used in experiments directly as biochemical analysis will be performed on extracted tissues and MEF cells.
Various organs from adult mice of different ages will be taken out for tissue sections and biochemical analysis.
We will establish MEF cells to complement our studies and reduce the number of mice needed.
We have planned our studies based on our previous experience to use the least amount of mice possible. Based on our calculations we will use a maximum of 556 mice in our studies.
The mice will not be used in experiments directly as biochemical analysis will be performed on extracted tissues and MEF cells.