Role of AQP4 and AQP9 in health and disease
1. Formål
Our laboratory has its focus on the role of aquaporin water channels. Aquaporin-4 (AQP4) is the predominant brain water channel. Aquaporin-9 (AQP9) is an aquaglyceroporin, also expressed in the brain. These aquaporins are important for brain water homeostasis and transport of solutes, and has evidence points to an serving an important role in several brain diseases such as stroke and Parkinsons disease. We have generated two transgenic mouse strains, one lacking AQP4 (AQP4-KO) and one lacking AQP9 (AQP9-KO).
The AQP4-KO animals lack the water channel molecule AQP4 which plays an important role in water and volume homeostasis in the brain and other organs such as muscle, heart, kidnet and probably gastro-intestinal tract.
The AQP9-KO animals lack the aquaglyceroporine AQP9 which is permeable to water as well as a
range of solutes including glycerol and urea. AQP9 is expressed in the brain and other organs such as liver. AQP9 likely plays an important role in glucogenesis of the liver and in Parkinsons disease in the brain.
In this project we will be breeding animals for several ongoing projects and do terminal experiments for harvesting organs. This is a continuation of previous projects and we have many years of experience with the experiments.
In the terminal experiments, the animals will decapitated or perfusion fixed and organs (most commonly the brain) will be dissected out and used for ex-vivo experiments. In some cases we will decapitate newly born pups and dissect the brain to make primary cell cultures.
2. Skadevirkninger
There are little or no harmful effects from these experiments. During the terminal experiments, the animals are deeply anesthetized with a ZRF-cocktail and feels no pain. During breeding and housing the experienced staff in the animal facility uses scoring sheet (attachment) for evaluation of pain and definition of human endpoint.
3. Forventet nytteverdi
We will be breeding animals and harvesting organs for several important projects in our research group. Maintaining our colony of these genetic lines are crucial. A continuation of projects that has been ongoing for many years is therefore decisive.
4. Antall dyr og art
We breed HZ x HZ in single or duo-breeding for the purpose of using WT littermates as controls and use both KO and HZ for experiments. For backcrossing we breed HZ x BL6-WT. We might also breed KO x KO and order BL6-WT controls from Janvier laboratories. For maintaining the genetic lines and/or breed for experiments we would keep between four to eight females breeding. An average female produces maybe 50 pups a year. This will produce between 200 and 400 pups per year. In addition we are ordering BL6-WT animals for breeding and experiments.
5. 3R
The effects of genetic mutations in brain diseases are investigated on a molecular level. To reduce usage we will accompany with other in vitro experiments or use cell lines. By dividing the brain into two hemispheres and regions we can use the same animal for many techniques.
We reduce to minimum number of animals for quantitative results.
Our laboratory has its focus on the role of aquaporin water channels. Aquaporin-4 (AQP4) is the predominant brain water channel. Aquaporin-9 (AQP9) is an aquaglyceroporin, also expressed in the brain. These aquaporins are important for brain water homeostasis and transport of solutes, and has evidence points to an serving an important role in several brain diseases such as stroke and Parkinsons disease. We have generated two transgenic mouse strains, one lacking AQP4 (AQP4-KO) and one lacking AQP9 (AQP9-KO).
The AQP4-KO animals lack the water channel molecule AQP4 which plays an important role in water and volume homeostasis in the brain and other organs such as muscle, heart, kidnet and probably gastro-intestinal tract.
The AQP9-KO animals lack the aquaglyceroporine AQP9 which is permeable to water as well as a
range of solutes including glycerol and urea. AQP9 is expressed in the brain and other organs such as liver. AQP9 likely plays an important role in glucogenesis of the liver and in Parkinsons disease in the brain.
In this project we will be breeding animals for several ongoing projects and do terminal experiments for harvesting organs. This is a continuation of previous projects and we have many years of experience with the experiments.
In the terminal experiments, the animals will decapitated or perfusion fixed and organs (most commonly the brain) will be dissected out and used for ex-vivo experiments. In some cases we will decapitate newly born pups and dissect the brain to make primary cell cultures.
2. Skadevirkninger
There are little or no harmful effects from these experiments. During the terminal experiments, the animals are deeply anesthetized with a ZRF-cocktail and feels no pain. During breeding and housing the experienced staff in the animal facility uses scoring sheet (attachment) for evaluation of pain and definition of human endpoint.
3. Forventet nytteverdi
We will be breeding animals and harvesting organs for several important projects in our research group. Maintaining our colony of these genetic lines are crucial. A continuation of projects that has been ongoing for many years is therefore decisive.
4. Antall dyr og art
We breed HZ x HZ in single or duo-breeding for the purpose of using WT littermates as controls and use both KO and HZ for experiments. For backcrossing we breed HZ x BL6-WT. We might also breed KO x KO and order BL6-WT controls from Janvier laboratories. For maintaining the genetic lines and/or breed for experiments we would keep between four to eight females breeding. An average female produces maybe 50 pups a year. This will produce between 200 and 400 pups per year. In addition we are ordering BL6-WT animals for breeding and experiments.
5. 3R
The effects of genetic mutations in brain diseases are investigated on a molecular level. To reduce usage we will accompany with other in vitro experiments or use cell lines. By dividing the brain into two hemispheres and regions we can use the same animal for many techniques.
We reduce to minimum number of animals for quantitative results.