Effect of eggshell membrane in aging mice
Egg shell membrane (ESM) is a side stream product from egg production commonly regarded as waste or low end product. The goal is to exploit such side stream products into high value products such as health promoting products. Previously it has been demonstrated that ESM has wound healing properties and anti-inflammatory properties. Anti-inflammatory action has been demonstrated in cell cultures, in a human intervention study and in various rodent models. The ageing process is hallmarked by an increased low-grade inflammatory state, which interferes with tissue homeostasis in many tissues including muscle and bone, with resulting loss in tissue mass. A potential target population for ESM is the elderly. The population aged 65+ is expected to double over the next 15 years, and age-related losses in bone and skeletal muscle mass and function present an extremely important public health issue.
We therefore aim to conduct an experiment in which we feed aging mice (14 months) for a period of 10 weeks three different doses of ESM to test whether this treatment ameliorate markers of inflammation and aging.
Number of animals applied for is 75.
These experiments can not be easily modelled or mimicked by in vitro studies. We do not expect that animals will experience pain or significant degree of discomfort. Nevertheless, animals are closely monitored on a daily basis to minimize discomfort. Number of animals in this study is calculated on the basis of power-analysis, literature searches and own experience. The animals are housed in individually ventilated cages, with controlled temperature, light regulation and humidity. Cages also contain running wheels, house and nesting material. The animals are looked after every day.
We therefore aim to conduct an experiment in which we feed aging mice (14 months) for a period of 10 weeks three different doses of ESM to test whether this treatment ameliorate markers of inflammation and aging.
Number of animals applied for is 75.
These experiments can not be easily modelled or mimicked by in vitro studies. We do not expect that animals will experience pain or significant degree of discomfort. Nevertheless, animals are closely monitored on a daily basis to minimize discomfort. Number of animals in this study is calculated on the basis of power-analysis, literature searches and own experience. The animals are housed in individually ventilated cages, with controlled temperature, light regulation and humidity. Cages also contain running wheels, house and nesting material. The animals are looked after every day.