Transgenerational, developmental and behavioral effects of natural POPs mixture and PFOS on zebrafish
This experiment will study the effects of exposure of POPs mixtures and PFOS on 3 generations of zebrafish (F0 exposed, F1 unexposed, F2 unexposed). For that purpose first a pilot study will take place using a POPs mixture already available at NMBU to determine the accumulation of chemicals in 5 dpf zebrafish. The experiment will start when zebrafish are 0 dpf and end at 5 dpf. 30 individuals will be used and this experiment will determine the concentrations of POPs that are going to be used in the main experiment. For the main experiments 350 individuals per replicate (5 replicates) will be exposed to 2 pre-determined concentrations (low and high) of POPs mixture and 2 concentrations of PFOS from 0-5 dpf. Afterwards there will be transported in clean water and the effect of exposure will be studied on the reproductive outcome (3-4 month of age, fertilization rate, hatching rate, gonad histology) and the development, morphology, behavior and survival. Reproductive outcome along with development and behavior will be studied also on the second and third generation of unexposed individuals.
During the course of these experiments the population parameters will be monitored (survival, growth rate, maturation rate, reproduction) to build population dynamics models to predict population-level responses.
The experiments are going to be performed on zebrafish (Danio rerio) and will provide information not only on the immediate effects of pollutants on the physiology of fish but also using ecological models will be able to predict the effects of pollutants on natural fish populations.
During the course of these experiments the population parameters will be monitored (survival, growth rate, maturation rate, reproduction) to build population dynamics models to predict population-level responses.
The experiments are going to be performed on zebrafish (Danio rerio) and will provide information not only on the immediate effects of pollutants on the physiology of fish but also using ecological models will be able to predict the effects of pollutants on natural fish populations.