A trial for investigation of parameters involved in efficacy of bacteria used in Biotic Enhancement treatment of Atlantic salmon
The study will define how bacteria in water relate to the Atlantic salmon . The first intention of the study is to determine optimal a) strain combination of three beneficial live innocuous bacterial cultures of Vibrio spp., b) exposure time, c) concentration, d) water salinity, e) temperature for maximum absorption of Vibrio spp. in Atlantic salmon.
The second intention of the study is to evaluate the relation between Atlantic salmon and a series of environmental and intestinal isolates of bacteria from marine environments.
The Atlantic salmon used will not have any negative impact from the study since blood sampling under anaesthesia is the only direct handling of the salmon that will be done. The fish will be euthanized after blood sampling and the duration of the experiment will only be minutes for each individual including exposure to the bacteria added to the water.
The scientific impact from the study will be important for future studies as a platform of understanding how bacteria interact with Atlantic salmon. For the general society the results will be used as a background for improving health and growth in Atlantic salmon farming leading to improved economic benefit with better animal welfare.
Two groups of fish will be compared with each other over all variables in the first part of the study. Group 1 have been in freshwater only. Group 2 are in seawater. This in order to investigate if absorbtion of live bacteria is most effective for fish in freshwater or fish already subjected to seawater two weeks prior to the experiment. Effect from exposure will be evaluated by drawing of blood after anesthetization. In total 344 Atlantic salmon in the range of 60 - 220 grams will be used in this study. The study design is novel and simple and will eliminate the need of holding fish in tanks for days and weeks in order to monitor the effect of bacteria that have been exposed to the fish in water.
The results from the study will be part of the documentation needed to show that the study treatment will increase fish health, fish weight and food conversion rate compared to untreated controls.
The second intention of the study is to evaluate the relation between Atlantic salmon and a series of environmental and intestinal isolates of bacteria from marine environments.
The Atlantic salmon used will not have any negative impact from the study since blood sampling under anaesthesia is the only direct handling of the salmon that will be done. The fish will be euthanized after blood sampling and the duration of the experiment will only be minutes for each individual including exposure to the bacteria added to the water.
The scientific impact from the study will be important for future studies as a platform of understanding how bacteria interact with Atlantic salmon. For the general society the results will be used as a background for improving health and growth in Atlantic salmon farming leading to improved economic benefit with better animal welfare.
Two groups of fish will be compared with each other over all variables in the first part of the study. Group 1 have been in freshwater only. Group 2 are in seawater. This in order to investigate if absorbtion of live bacteria is most effective for fish in freshwater or fish already subjected to seawater two weeks prior to the experiment. Effect from exposure will be evaluated by drawing of blood after anesthetization. In total 344 Atlantic salmon in the range of 60 - 220 grams will be used in this study. The study design is novel and simple and will eliminate the need of holding fish in tanks for days and weeks in order to monitor the effect of bacteria that have been exposed to the fish in water.
The results from the study will be part of the documentation needed to show that the study treatment will increase fish health, fish weight and food conversion rate compared to untreated controls.