Breeding of CD100 gene modified lines and controls

The purpose of this application is to keep CD100 KO, KI and control mice running in our lab, and provide gene modified animals and controls for other experiments. Since no intervention except ear tag will be performed on them, there is very little expected distress for the animals. The breeding will be the base for other animal experiments using these lines. We plan 500 CD100KO(B6N background), 500 CD100KI(B6J background), 300 B6N controls and 300 B6J controls for 3 years. Breeding pairs will set to the the smallest size when there is no experiment for gene modified mice.