Immunization of salmon parr to obtain plasma for ELISA
The purpose of this experiment is to obtain plasma from immunised fish to develop an ELISA for Canadian Tenacibaculum maritimum isolates. This work will contribute to the development of a vaccine against the bacteria. Mouthrot infections caused by T. maritimum have a significant impact on the industry in Western Canada due to the need for antibiotics to treat the disease. This experiment aims to further the knowledge of this disease to allow for future development of control measures.
A total of 165 Atlantic salmon will be a part of the experiment, all of which will be immunized with a vaccine (inactivated bacteria) based on one of the T. maritimum isolates from Western Canada. The objectives of the experiment cannot be carried out without the use of laboratory fish. Selection of the number of animals is based on the minimum needed to obtain enough plasma to perform ELISA testing.
A total of 165 Atlantic salmon will be a part of the experiment, all of which will be immunized with a vaccine (inactivated bacteria) based on one of the T. maritimum isolates from Western Canada. The objectives of the experiment cannot be carried out without the use of laboratory fish. Selection of the number of animals is based on the minimum needed to obtain enough plasma to perform ELISA testing.